The process of ethanol production starts by milling a feed stock followed by the addition of dilute or fungal amylase (enzyme) from Aspergillus to break down the starch into fermentable sugars. Yeast is then added to convert the sugars to ethanol which is then distilled off to obtain ethanol which is upto 96 percent in concentration. Ethanol and biodiesel, represents the first generation of biofuel technology. Ethanol is often used as a fuel, mainly as a biofuel additive for gasoline