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In E.coli, three enzymes β- galactosidase, permease and transacetylase are produced in the presence of lactose.

Explain why the enzymes are not synthesized in the absence of lactose.

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In the absence of lactose, the repressor protein binds to the operator and prevents the transcription of structural gene by RNA polymerase, hence the enzymes are not produced. However, there will always be a minimal level of lac operon expression even in absence of lactose.

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