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Which of the following limits the number of trophic levels in a food chain? 

A. decrease in energy at higher trophic levels 

B. deficient food supply 

C. polluted air 

D. water

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As we know only 10 % of the total energy available at any trophic level is transferred to the next higher trophic level. So, as we move up the higher trophic level, the energy keeps on decreasing. Therefore, decrease in energy at higher trophic limits the food chain.

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