# A given protein with a molecular weight of 20,000 daltons containing 5,4,3,2, and 1 charges, is subjected to mass spectrometry.

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A given protein with a molecular weight of 20,000 daltons containing 5,4,3,2, and 1 charges, is subjected to mass spectrometry. Find the sequence of protein ions detected by the mass spectrometer.

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$\mathrm{\frac{m}{z}=M+\frac{nH}{n}}$

$\mathrm{\frac{m}{z}=\frac{20,005}{5}=4001}$

$\mathrm{\frac{m}{z}=\frac{20,004}{4}=5001}$

$\mathrm{\frac{m}{z}=\frac{20,003}{3}=6668}$

$\mathrm{\frac{m}{z}=\frac{20,002}{2}=10,001}$

$\mathrm{\frac{m}{z}=\frac{20,001}{1}=20,001}$

Sequence— It detects protein ions at $\mathrm{\frac{m}{z}}$= 4001, 5001, 6668, 10001 and 20,001 respectively.