Question

A given protein with a molecular weight of 20,000 daltons containing 5,4,3,2, and 1 charges, is subjected to mass spectrometry. Find the sequence of protein ions detected by the mass spectrometer.

Answer 1

\(\mathrm{\frac{m}{z}=M+\frac{nH}{n}}\)

\(\mathrm{\frac{m}{z}=\frac{20,005}{5}=4001}\)

\(\mathrm{\frac{m}{z}=\frac{20,004}{4}=5001}\)

\(\mathrm{\frac{m}{z}=\frac{20,003}{3}=6668}\)

\(\mathrm{\frac{m}{z}=\frac{20,002}{2}=10,001}\)

\(\mathrm{\frac{m}{z}=\frac{20,001}{1}=20,001}\)

Sequence— It detects protein ions at \(\mathrm{\frac{m}{z}}\)= 4001, 5001, 6668, 10001 and 20,001 respectively.