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What is the effect of increase in substrate concentration on the rate of enzymatic reaction ?

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Increase in substrate concentration increases the rate of reaction. 

The enhanced rate is due to two factors : 

(i) Occupation of one and more active sites by the substrate molecules. 

(ii) Higher number of collisions between substrate molecules. The rise in velocity is quite high in the beginning but it decreases progressively with the increase in substrate concentration.

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