The factors which set the limit of cell size or volume are:
(i) Nucleo-cytoplasmic ratio which determines the range of control of metabolic activities by nucleus.
(ii) Ability of oxygen and other materials to reach every part of cell.
(iii) Ability of waste products to pass to the outside.
(iv) Rate of metabolic activity.
(v) Rate of surface area to the volume of the cell.
