Question

Observe the given graph a and

1. State how temperature and pH influences enzyme activity. 

2. Does the substrate concentration influence enzyme activity. If so, how?

Answer 1

1. Each enzyme shows its highest activity at a particular temperature. This is called optimum temperature. Low temperature or high temperature reduces the enzyme activity. Same as, there is an optimum pH for enzyme activity. Deviation from this pH reduces the enzyme activity.

2. With the increase in substrate concentration, the velocity of the enzymatic reaction increase at first, as the substrate concentration continues to increase, the increase in the rate of reaction begins to slow down and finally no further rise in velocity occurs. This is because there is no free enzyme molecule to bind additional substrate molecule.