Question

The restriction enzymes that are used in construction of recombinant DNA molecule are endonucleases which cut the DNA at ‘specificrecognition sequence’. What would be the disadvantage if they would not cut the DNA at specific-recognition sequence?

Answer 1

If the restriction enzymes would cut DNA at random sites instead of at specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky ends, construction of recombinant DNA molecule would not be possible.