Question

Explain the roles of the following with the help of an example each in recombinant DNA technology?
(a) Restriction Enzymems(b) Plasmids

Answer 1

Out breeding : out breeding is the breeding fof he unrealted animals which may be between individuals of the same breed (but having no common ancestors) or between different breeds (corss breeding or different species (intterspecific hybridisation )
out crossing : This is the practive oc mating of animals within the same breed but having no common ancestors on either side of their pedigree up up to 4-6 generations.The off springs of such a mating is known as an out cross .It is the best breeding method for animlas that are below average in productivity in milk production growth rate in beef cattle ect .A single out corts helps to voercome the inbreeding depression

Answer 2

Restriction enzymes, also known as restriction endonucleases, are enzymes that cut a DNA molecule at a particular place. They are essential tools for recombinant DNA technology. The enzyme "scans" a DNA molecule, looking for a particular sequence, usually of four to six nucleotides. Once it finds this recognition sequence, it stops and cuts the strands. Example: EcoRI, HindIII.

Plasmids are extrachromosomal self-replicating bacterial DNA which is used as a cloning vector in recombinant DNA technology. Example - pBR322