Preparation of glucose from sucrose (cane sugar) :
Laboratory method.
Glucose is prepared in the laboratory by hydrolysis of sucrose by boiling it with dilute hydrohloric acid or dilute sulphuric acid for about two hours.
On hydrolysis, sucrose gives one molecule of glucose and one molecule of fructose.
Alcohol is added during cooling to separate glucose and fructose since, glucose is almost insoluble in alcohol, hence it crystallizes out first. Fructose remains in the solution as it is more soluble than glucose.
Crystals of glucose are separated out by filtration and purified by recrystallization.
