Question

Which of the following restriction enzymes produces blunt ends?

(a) Sa/ I 

(b) Eco RV 

(c) Xho 

(d)Hind III

Answer 1

Correct option (b) Eco RV 

Explanation:

Eco RV is a type II restriction endonuclease isolated from strains of E. coli. It creates blunt ends. The enzyme recognises the palindromic 6-base DNA sequence and makes a cut at vertical line. The blunt ends are formed as