Correct Answer - C
Coenzyme/cofactors are organic substances (often vitamins) which are loosely attached with apoenzymes. A holoenzyme is a conjugated enzyme. It has a proteinaceous part called apoenzyme and a non-proteinaceous part called cofactor.
`underset("(conjugated enzyme)")("Holoenzyme")hArrunderset("non-protein part")underset("+ Cofactor")underset("(Protein part)")("Apoenzyme")`
Cofactor is very necessary for the activity of holoenzyme.
Cofactors can be separated from enzyme by dialysis. Cofactors may be inorganic (i.e. metal activators) or organic (i.e. coenzymes and prosthetic group).