Question

Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.

Answer 1

E. coli cloning vector pBR322 showing restriction sites (Hindi III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I), ori and antibiotic resistance genes `(amp^(R) and tet^(R))`. Rop codes for the proteins involved in the replication of the plasmid.