i. Name the chromatographic technique involved. ii. From the developed chromatogram, state which has the highest and which has the lowest Rf value?

Question

Based on the above diagram, answer the following questions :

i. Name the chromatographic technique involved.

ii. From the developed chromatogram, state which has the highest and which has the lowest R_f value?

iii. Based on the TLC, which component would elute out at the end of a column chromatography?

iv. Mention two applications of TLC method.

Answer 1

i. Thin layer chromatography

ii. Based on the developed chromatogram, spot ‘x’ has the highest R_f value while spot ‘z’ the lowest R_f value.

iii. Based on the TLC, spot ‘z’ being strongly adsorbed will elute at the end of a column chromatography.

iv. Applications of TLC are :

• Separation of plant pigments from its mixture.
• Separation of impurities from a given organic compound.
• Separation of different amino acid.