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What does “T” refers to in Tissue culture flask (T-Flask)?

(a) Total volume of the flask

(b) Total weight of the flask

(c) Total surface area of the flask

(d) Total mass of the flask

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Correct choice is (c) Total surface area of the flask

Explanation: The “T” referred to the total surface area of the flask that was available for cell growth: thus a T-25 flask had a 25cm^2 growth area. By the 1960s, straight neck T-flasks was available molded from polystyrene that was treated to enhance cell attachment.

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