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Which of the following is used in PAGE to prevent the mixing of the sample with running buffer?

(a) ethanol

(b) methanol

(c) chloroform

(d) sucrose

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Right choice is (d) sucrose

The best I can explain: For polyacrylamide gel electrophoresis (PAGE), the protein samples are prepared in a solution of sucrose or glycerol to provide density. This prevents the mixing of protein samples with the running buffer.

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