It is critical to fix cytology specimens immediately after collection for proper preservation of the cellular components. It is important that no air-drying occurs prior to fixation. If a smear is already air-dried it should not be put in an alcohol fixative. Please note on the requisition if the slide (s) being submitted are fixed or air-dried. Properties of a good cytological fixative:
• It should not excessively shrink or swell cells.
• It should not distort or dissolve cellular components.
• It should help preserve nuclear details.
• It should improve optical differentiation and enhance staining properties of the tissues and cell components.
