The various steps involved in the production of transgenic organisms are :
1. Identification and separation of desired gene.
2. Selection of a vector (generally a virus) or direct transmission.
3. Combining the desired gene with the vector.
4. Introduction of transferred vector into cells, tissues, embryo or mature individual.
5. Demonstration of integration and expression of foreign gene in transgenic tissue or animals. Transgenic animals such as mice, rat, rabbit, pig, cow, goat, sheep and fish have been produced.
