Sarthaks Test
0 votes
in Biotechnology by (47.5k points)
closed by

Explain various steps involved in a recombinant DNA technology experiment. Name any two molecular biologists who helped to create the first r-DNA molecule.

1 Answer

+1 vote
by (46.4k points)
selected by
Best answer

Steps involved: 

1. Isolation of a DNA fragment containing a gene of interest that needs to be cloned (called as insert). 

2. Generation of a recombinant DNA (rDNA) molecule by insertion of the DNA fragment into a carrier DNAmolecule called vector (e.g. plasmid) that can self replicate within a host cell. 

3. Transfer of the rDNAinto an E. coli host cell (process called transformation). 

4. Selection of only those host cells carrying the rDNA and allowing them to multiply thereby multiplying the rDNA molecules. 

Paul Berg, Herbert Boyer, Annie Chang and Stanley Cohen.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.