Question

A group of students came to know about recombinant DNA technology. They want to know how scientists can produce a new desired product using rDNA technology. Can you give them an idea about the important steps that are involved in this process?

Answer 1

Recombinant DNA technology involves several steps. 

They are

(1) Isolation of the Genetic Material (DNA)

(2) Cutting of DNA at Specific Locations

(3) Amplification of Gene of Interest using PCR 

(4) Insertion of Recombinant DNA into the Host Cell Organism

(5) Obtaining the Foreign Gene Product

(6) Down stream processing