Question

Some foreign DNA fragment is attached to Cla I site of pBR 322. This recombinant vector in used to transform Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vecotr will
A. Grow on both, tetracycline and ampicillin containing media
B. Not grow on either tetracycline containing or ampicillin containing media
C. Grow on tetracycline but not on ampicillin containing medium
D. Grow on ampicillin but not on tetracycline containing medium

Answer 1

Correct Answer - A
Some foreign DNA fragment is attached to Cla-I site of pBR322. This recombinant vector in used to transition Escherichia coli. Then on two different media, one containing ampicillin and the other containing tetracyline. The transformed cells containing the recombinant vector will grow on both, tetracyline and ampicllin containing media because Cla-I site is neither present on `amp^(R)` gene nor on `"tet"^(R)` gene