Question

Some foreign DNA fragment is attached to Cla I site of pB322. This recombinant vector is used to transit Escherichia coli host cells. The cell subjected to transformation are plated on two different media-one containing ampicillin and the other containing tetracycline. THe transformed cells containing the recombinant vector
A. will grow on both tetracycline containing and ampicillin containing media
B. will not grow on either tetracycline containing or ampicillin containing media
C. will grow on tertracycline, but not on ampicillin containing medium.
D. will grow on ampicillin, but not on tetracycline containing medium

Answer 1

Correct Answer - d
The transformed cell will grow on ampicillin but not on tetracycline. This is because the foreign DNA fragment is attached to Cla I site, which is present in the region of `tet^(R )` gene. Thus, it cause disintegration of `tet^(R )` gene.