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During gene cloning, the enzyme used to join and insert DNA within plasmid is

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During gene cloning, the enzyme used to join and insert DNA within plasmid is
A. DNA ligase
B. restriction endonucleases
C. alkaline phosphatase
D. exonuclease
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In molecular biology, DNA ligase is a special type of enzyme that is used to seal the double-stranded breaks, i.e. breaks in both complementary strands of DNA or is used to join insert DNA with the plasmid vector.
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