Question

Few restriction enzymes break the phosphodiester bond in such a manner that single stranded overhang ends are generated in the DNA strand. EcoRI is one such a restriction enzyme.

(a) Write the sequence for restriction site for enzyme EcoRI. Give a name to the type of ends generated here. Are all the restriction sequences palindromic in nature?

(b) Explain about any two vectorless methods that allow DNA to enter host cells.

(c) Why is small size desirable in a cloning vehicles?

Answer 1

(a) Restriction site of EcoRI is 5’-GAATTC-3’ 

The ends generated will be called sticky.

No, all the Restriction sequences may not be palindromic.

(b) Microinjection can inject foreign DNA into plant and animal cells

Biolistics makes use of particle gun to bombard gold coated DNA Into cells.

(c) Small size of vector facilitates entry of recombinant molecules into the host cells.